The purpose undertakes study to the chemical composition of chickweed. The method such as methodological coloring chart undertakes depart rarefied, change property and structure of spectral data appraisal according to manage. The result departs and appraisal 7 compound, it is Chinese rhubarb element respectively (Ⅰ ) , aether of armour of Chinese rhubarb element (Ⅱ ) , chinese rhubarb element - 8- armour aether (Ⅲ ) , 26 alkyl are mellow (Ⅳ ) , β - cereal steriod is mellow (Ⅴ ) , carrot glucoside (Ⅵ ) , hill Nai is phenolic - 3, 7-O- α - glucoside of L- buckthorn candy (Ⅶ ) . Ⅰ ～ Ⅳ and Ⅶ all are conclusion compound to share from inside this plant first, in belonging to a plant for chickweed, depart first get.
The purpose discusses element of Mongolian oak skin the effect of prevention and cure that chronic to animal experiment sex kidney declines. The method gives experimental bandicoot raise to caused chronic kidney in order to contain gland purine feed to decline in Feburary Ig of; interpose group gives Mongolian oak Pi Su (cure of 42d) of × of D-1 of · of 100mg · Kg-1. The essence when observing condition of health of each groups of animals and mortality; end says surviving bandicoot weight, kidney is weighed and law of drying of; of computational kidney index determines pathology of kidney of travel of groovy method of; of kidney organization water content checks; with Beckman full automatic biochemical analyzer determines serum kidney result is relevant biochemical index. Result and kidney decline contrast group quite, mortality of interpose group animal is reduced significantly () of ﹪ of 20 ﹪ Vs84, surviving rat weight increases significantly (＜ 0.001) of 14)g(P of ± of 240 ± 22)g vs(109, nephritic index and kidney organization water content all are reduced significantly [(﹪ of 1.25) of Vs(78.24 ± of ﹪ of 5.08 ± 0.89) and (﹪ of 0.90) of Vs(79.29 ± of ﹪ of 12.63 ± 2.94) ] (P ＜ 0.001, p ＜ 0.05) , change of nephritic pathology injury is apparent lighter, related serum kidney result biochemical index (BUN, CREA, URIC and GLU) change are reduced significantly [(20.9 ± 5.7) , (82.8 ± 51.5) , (155.9 ± 24.3) , (14.0) of ± of 11.79 ± 1.54)vs(78.7, (246.7 ± 55.9) , (108.7 ± 31.5) , (8.14 ± . Element of skin of conclusion Mongolian oak is chronic to bandicoot experiment sex kidney declines have effect of remarkable prevention and cure.
Composition of cholic acid of inject fluid Central Africa is opposite purpose research Qing Kailing cholic acid (CA) and different deoxygenate cholic acid (the influence that acting kinetic process reachs HDCA) medicine to distributing in main internal organs of the body, open the pharmacodynamics action of quick injection fluid in order to clarify clear. Methodological test animal divides 2 groups, qing Dynasty opens quick injection fluid (the CA/HDCA form that group of 10ml · Kg-1) and sheet use same dose, small rat is abstinence before giving drug can’t help water 12h, orbit minim collects blood 20 μ L, after oscillation of direct precipitation albumen centrifugal, take Qing Dynasty to detect into appearance. Metage of filter paper blot is used after taking heart, lung, kidney, head to clean, use 4 divide evenly of 1 ∶ oar hind is centrifugal take Qing Dynasty, the processing technique detects into appearance with hematic sample. Color atlas of use fluid photograph / usage of mass spectra couplet detects blood and the content that organize the CA in sample and HDCA. Result CA and HDCA are mixed in complete blood the limits of medicaments chroma linear in the organization is L-1 of · of μ G of 4 ～ 4 000, linear relationship is good (R ＞ 0.999) , lowest detects chroma is L-1 of · of 2 μ G, daytime and the RSD of the precision inside day all ＜ 5 ﹪ . Acting kinetic parameter mixes the drug that compares the CA when medicine made of two or more ingredients counteracts sheet to use and HDCA basically organizing (heart, lung, kidney, head) distributing mediumly, β of the AUC that Qing Dynasty opens the CA in quick injection fluid and HDCA, MRT, T1/2, V all when sheet of apparent prep above is used (P ＜ 0.05) , when CL is used under sheet apparently (of the CA in fluid of inject of Qing Kailing of P ＜ 0.05); and HDCA distributing apparent excel sheet uses CA/HDCA, CA and HDCA are in the distributinging characteristic in the organization: CHead of C of ＞ of heart of C of ＞ of kidney of lobar ＞ C. Conclusion this methodological sensitivity is tall, particularity is good, composition of cholic acid of Central Africa of fluid of inject of Qing Kailing of; of research of the process inside the body that applies to cholic acid apperception to close matter can lengthen cholic acid composition the distributinging;CA in the organization and HDCA are in the detention time inside body and composition of stimulative cholic acid low specification of the chroma in the head its pass through hematic head not easily.
The purpose is built Chinese the hemolysis of fluid of inject of element of armour of the root of fangji and agglomeration examination law. Law of the tube outside methodological body is used ” Chinese pharmacopoeia ” hemolysis of ” of rudder of application of law of injection security examination and agglomeration check 2005 edition Chinese traditional medicine law ” , consult bureau of supervisory processing of national provision medicines and chemical reagents is made ” excitant, irritability and haemolysis sex study chemical medicaments instructor principle ” relevant content. The result is 5.0mg · Ml-1 when chroma, when bulk is 0.3ml, inside 37 ℃ 3h, chinese incorrect hare red blood cell produces juice of inject of element of armour of the root of fangji haemolysis and agglomeration action. Conclusion can choose chroma to be 5.0mg · Ml-1, bulk is 0.3ml Chinese solution of sodium chloride of physiology of fluid of inject of element of armour of the root of fangji, the method that presses a regulation undertakes haemolysis and agglomeration are checked.
Water of root of beans of purpose research north carries content to be opposite velar fluidity and blood affect small rat red blood cell groovily. The method gives chroma to be respectively to 3 groups of small rat 1, 0.5, the water of boreal beans root of 0.25g · Ml-1 promotes thing, give medicine 3 weeks continuously, set group of solution of physiology sodium chloride to regard as contrast. Use hematic cell analyzer to undertake haemal composition determines, use law of DPH fluorescent bougie to determine fluidity of film of red blood cell. Result blood is groovy water of root of beans of the north in detecting promotes thing 3 dosage group, compare leucocyte amount with group of solution of physiology sodium chloride (WBC) has downtrend, chroma group has volume of cell of average blood of significance difference; in (content of MCV) , haemoglobin (HGB) reduces; significantly low, medium chroma group red blood cell (RBC) has ascendant tendency, low density group has plaque of statistical difference; (PLT) lifts significantly (P ＜ 0.01) . Boreal beans root is low, medium chroma team can add liquidity of film of red blood cell significantly, reduce small viscosity, and the effect of high concentration group is not apparent. Conclusion is certain water of root of beans of pH indicator north carries content to be able to add liquidity of red thin film, reduce haemal viscosity thereby, improve hematic current to change index.
The purpose builds law of color atlas of concentrated juice photograph to determine the method of content of acerbity ester of ammonia of pleasant of element of mildew of sulphone of hydrochloric acid armour. The method is efficient chromatographic column of; of law of fluid photograph color atlas: XDB-C18 column(250mm × 4.6mm, photograph of flow of 5 μ M); : 0.2 ﹪ phosphoric acid aqueous solution of 2 hydrogen ammonium - second nitrile (85 ∶ 15); detects wavelengh: 224nm. Result linear limits: 0Ml-1 of · of 1.004 6mg of.000 980 ～ , r=0.999 9; reclaims on average rate for 100.4 ﹪ , RSD is conclusion of 0.27 ﹪ ; this method result is accurate, the operation is handy, the content that applies to this medicaments measures calm analysis; to use HPLC method the influencing factor of stability of aqueous solution of acerbity to ammonia of pleasant of element of mildew of sulphone of hydrochloric acid armour ester undertakes study, the PH of solution is worth ammonia of pair of pleasant of element of mildew of sulphone of hydrochloric acid armour impact of stability of acerbity ester aqueous solution is the biggest, the stablest PH range is PH1 ～ 3.
Grain of licorice of Lou of purpose observation melon is pneumonic to flu virus the influence of small rat. The method gets used to individual plant via lung of armour of Asia of virus of nose inspiratory flu rat with small rat (FM1) is duplicate sex of small mouse virus is pneumonic model, have treatment with grain of melon Lou licorice, install Libaweilin to contrast group, observe its are pneumonic to virus index of the mortality of small rat, weight, lung and virus blood coagulate a spent influence. Group of treatment of grain of licorice of result melon Lou relatively model group mortality is reduced apparently, live on average day number is lengthened apparently, weight drops get clear improvement, lobar index and blood coagulate drop degree reduce apparently. Conclusion heats up expectorant, detoxify to help the grain of melon Lou licorice that is a law up to treat virus of small rat flu with Qing Dynasty pneumonic curative effect is positive.
The purpose studies board La Gen is different the action of anti-current feeling virus that draws position, the sieve is singled out board the active position of La Genkang virus. The method uses the culture method outside gallinaceous embryo body to be opposite board different extraction place undertakes La Gen the efficiency that fights flu virus determines. Total water of result board blue root carries fluid (Ⅰ ) , chloroform extraction place (Ⅲ ) have prevent action with way and different way, fourth mellow extractive place (Ⅴ ) have different way to prevent action, freeze-dry water carries fluid (Ⅶ ) have prevent action with the way, these 4 place all have the active that fights flu virus. Place of extraction of chloroform of conclusion board blue root, fourth mellow extractive place is board the active position of La Genkang virus.
The purpose studies Lian Huaqing fights the action of armour flu virus outside body of capsule of acute communicable diseases. The method makes masculine gender contrast with Libaweilin medicaments, use the inhibition outside retaining the put oneself in another’s position that takes law of cellular crystallization violet coloring to determine to spend capsule of clear acute communicable diseases to differ to shed feeling virus H3N2 to armour person to formulary type repeatedly and meantime effect impact. As a result capsule of Lian Huaqing acute communicable diseases has much link to fight the action of virus of armour person flu: A surname bowstring is planted circle of ⒍ of chart Yong deceive ” regret falls ⒁ of deceive of Yong of cerebral し thunder is planted cast aside ” blown away by wind of Yong of spoon of crucian carp of thick garden of mother of Shan of regret glue makes an appointment with pa benzene by low twinkling ” lens mires Yong? the half the number of these 4 kinds of action is significant chroma (EC50) , 0.031, 0.051, 0.050 G/mL, the effect that gives formulary type anti-current in order to prevent feeling virus is the most powerful. Along with the extension of medicaments effect time, the poison of low density disease-resistant that spends capsule of clear acute communicable diseases repeatedly is businesslike show reduce a current, when Gao Nong is spent (its fight ≥ 0.031 G/mL) virus ability is basic and changeless. Spend capsule of clear acute communicable diseases to be able to reduce the infection sex of virus apparently repeatedly at the same time. Conclusion has even capsule of the acute communicable diseases that spend Qing Dynasty action of virus of armour person flu is fought outside clear put oneself in another’s position.
The purpose discusses what head of hematic Yu sex is short of hematic model to establish a method. The method uses the method with loose rice of a place of strategic importance of intramuscular injection ground to build model of bandicoot blood Yu, daily note rice of ground a place of strategic importance at flesh of inside of bandicoot hind leg loose solution 0.2 Mg/kg, successive inject 10 D. When hematic Yu model is successful, anaesthetic animal is detached total artery of two side neck makes ligation art, build a head to be short of hematic model, blank group and among them group of a group of models uses method of make use of sb. Result and blank group are compared, viscosity of complete blood of surgery team and form of art of make use of sb is worth and the change of value of TT, PT, APTT has significance sense (P ＜ 0.01); and blank group and surgery team are compared, enzymatic vigor of content of the ATP vigor that head of bandicoot of false surgery team organizes, lactic acid and lactic acid dehydrogenation, N0 and NOS content, SOD and MDA content, Glu and T-AA content, ET and CGRP content change have significance sense (P ＜ , the complete blood viscosity that bolus of smooth, China Tuo give somebody a new lease on life adds up to a head to be short of hematic model bandicoot to hematic Yu, blood condenses Nimode enzymatic vigor of content of vigor of time, ATP, lactic acid and lactic acid dehydrogenation, N0 and NOS content, SOD and MDA content, Glu and. Conclusion uses afore-mentioned methods to be able to build head of hematic Yu sex successfully to be short of hematic bandicoot model.