Herbal Medicine

December 4, 2007

Alcohol of white black false bellebore forms protective action Protective Effect Of Resveratrol On Human Keratinocytes After UVB Irradiation of the cell to cutin of later generations of illuminate of medium wave ultraviolet ray

Filed under: New Herbal Pharmacology — Tags: , , , , , , , — admin @ 8:00 am

The purpose discusses alcohol of white black false bellebore to be opposite medium wave ultraviolet ray (cutin of UVB) illuminate later generations forms a cell (HaCaT cell) protective action of injury and its are likely mechanism. The method applies MTT experiment to observe to pH indicator differs inside limits of 0.001 ~ 0.5 Mmol/L the influence with proliferous to HaCaT cell alcohol of white black false bellebore. After using cell of U-VB(30 mJ/cm2) illuminate HaCaT, join different chroma instantly (0.01, the alcohol of white black false bellebore of 0.1 mmol/L) undertakes intervention, set blank group and UVB to contrast at the same time group, detect with MTT law respectively cellular hyperplasia ability, law of the amine that use hydroxide, colorimetric law, TBA law detects the cell exceeds oxide branch to change enzymatic (peptide of pleasant of Guang of SOD) , cereal crosses oxide enzymatic (GSH-Px) active and third 2 aldehyde (MDA) content, electric lens observes the cell exceeds the change of small structure. Mellow effect of white black false bellebore of different pH indicator is normal as a result after HaCaT cell, cellular hyperplasia ability does not have apparent difference (P > 0.05) . Hyperplasia of cell of the HaCaT after alcohol of white black false bellebore can promote UVB illuminate, increase cellular SOD and GSH-Px active, reduce MDA content, and high concentration group is inferior chroma group action is more remarkable (P < 0.05) . Electric lens shows alcohol of white black false bellebore can reduce UVB to exceed the loss of small structure to HaCaT cell. Alcohol of white black false bellebore of different pH indicator is opposite conclusion hyperplasia of normal HaCaT cell rings without clear picture. Alcohol of white black false bellebore is right outside body the HaCaT cell after UVB illuminate has protective effect, its mechanism likelihood and raise oxidation enzymatic active, cleared freedom base about.

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