The purpose discusses alcohol of white black false bellebore to be opposite medium wave ultraviolet ray (cutin of UVB) illuminate later generations forms a cell (HaCaT cell) protective action of injury and its are likely mechanism. The method applies MTT experiment to observe to pH indicator differs inside limits of 0.001 ～ 0.5 Mmol/L the influence with proliferous to HaCaT cell alcohol of white black false bellebore. After using cell of U-VB(30 mJ/cm2) illuminate HaCaT, join different chroma instantly (0.01, the alcohol of white black false bellebore of 0.1 mmol/L) undertakes intervention, set blank group and UVB to contrast at the same time group, detect with MTT law respectively cellular hyperplasia ability, law of the amine that use hydroxide, colorimetric law, TBA law detects the cell exceeds oxide branch to change enzymatic (peptide of pleasant of Guang of SOD) , cereal crosses oxide enzymatic (GSH-Px) active and third 2 aldehyde (MDA) content, electric lens observes the cell exceeds the change of small structure. Mellow effect of white black false bellebore of different pH indicator is normal as a result after HaCaT cell, cellular hyperplasia ability does not have apparent difference (P ＞ 0.05) . Hyperplasia of cell of the HaCaT after alcohol of white black false bellebore can promote UVB illuminate, increase cellular SOD and GSH-Px active, reduce MDA content, and high concentration group is inferior chroma group action is more remarkable (P ＜ 0.05) . Electric lens shows alcohol of white black false bellebore can reduce UVB to exceed the loss of small structure to HaCaT cell. Alcohol of white black false bellebore of different pH indicator is opposite conclusion hyperplasia of normal HaCaT cell rings without clear picture. Alcohol of white black false bellebore is right outside body the HaCaT cell after UVB illuminate has protective effect, its mechanism likelihood and raise oxidation enzymatic active, cleared freedom base about.
The expression that the purpose discusses 2(AQP2) of albumen of nephritic water passageway and metabolization of fluid of water of diabetic nephrosis bandicoot are unusual reach lustre the mechanism of action of prevention and cure of yellow decoct agent. The method adds small dose STZ with stimulation of feed of tall fat of high in syrup model of duplicate diabetic bandicoot, random cent contrasts for model group, positive group, lustre yellow decoct group, set normal group additionally. Immune systematism learns to detect AQP2 is in kidney of each groups of bandicoot distributings mediumly the expressive;RT-PCR method that reachs protein detects the expression of AQP2mRNA of each groups of bandicoot, and with β - Actin is relatively mensurable. Group of result lustre yellow decoct and positive contrast albumen of make water of group of hollow blood sugar, 24 h is excreted rate relatively model group all drops significantly. Kidney immunity group is changed show: AQP2 basically provides expression in nephritic gather. Compare with model group, lustre the expression of the nephritic AQP2 albumen of animal of yellow decoct group, AQP2mRNA all is reduced apparently (P ＜ 0.05) . The AQP2 in organization of kidney of bandicoot of conclusion model group conveys grow in quantity, AQP2 grow in quantity may participate in the formation of diabetic much make water. Lustre yellow decoct agent to water passageway albumen the expression of 2 has reduce effect, this may be its adjust the mechanism of action of water fluid metabolization, also be its prevent the foundation that treats diabetic nephrosis.
Purpose observation is unripe, fry definitely the pharmacodynamics action of pine torch, compare both difference. The method uses 4 chloridize carbon to incur loss of liver of small rat acute, determine; of content of serum ALT, AST measures Yiwen to think of La Zaizheng to often be mixed the character of excrement and urine that small intestinal of costive small rat advances distance; to observe costive small rat, defecate is preclinical with defecate amount. The result is unripe, fry definitely pine torch all has the distinct effect that protect liver, can reduce level of serum ALT, AST, both quite, fry definitely pine torch defends liver action strong at be born definitely pine torch; is unripe, fry definitely pine torch all can increase to be mixed normally the small intestine of costive small rat presses action, improve the character of excrement and urine of costive small rat, shorten the defecate of costive small rat is preclinical, increase defecate number, both compare action to comparative. Conclusion is unripe, fry definitely pine torch all has protect liver and embellish bowel aperient action. Protecting liver to fall enzymatic respect, fry definitely pine torch is strong at be born definitely pine torch; is in embellish bowel is aperient respect, unripe definitely pine torch and fry definitely pine torch action comparatives.
The purpose discusses clear gold to must give birth to a relevant element that controls lung cancer cell to grow mechanism. The method uses immune systematism to learn coloring (SABC) method detects clear gold must give birth to a serum that contain drug to process cancer cell of gland of later generations lung enzymatic active of bead of individual plant end and wither die the expressive level of relevant gene Bcl-2, Bax. As a result clear gold must give birth to a 72 h of action of the serum that contain drug, cancer cell of person lung gland carries an enzymatic active to be restrained apparently, can reduce Bcl-2 albumen positive at the same time cellular coloring rate, on rate of coloring of cell of positive of tone Bax albumen. Conclusion clear gold must be born piece carry an enzymatic active through restraining cancer cell of person lung gland, adjust the expression of Bcl-2 and Bax albumen, play fights tumor action.
The purpose observes 6 flavour are mixed in come loose the influence of hormone of bowel of pair of gastric bowel motivation, stomach and its are likely action mechanism. Method with sweet arenaceous 6 gentleman bolus is contrast group, in observing 6 flavour mix different dosage, come loose to be advanced to small rat small intestine than, the influence; that path of bowel of rate of the remain inside the stomach, complete stomach drives athletic time chooses 200 ﹪ pieplant great fry agent is duplicate model of bandicoot lienal empty, use radiative immunity law to detect different dosage 6 flavour and in come loose element of pair of secrete of stomach of lienal empty bandicoot (GAS) , stomach uses element (peptide of hemal and MTL) , active bowel (the influence of the gastric bowel hormone such as VIP) . As a result 6 flavour are mixed in medicinal powder each dosage group and blank contrast group quite, have raise small rat small intestine to advance apparently than, reduce the remain inside the stomach rate, shorten the action that path of complete stomach bowel presses athletic time (P ＜ 0.05 or P ＜ 0.01) , high dose group comes loose in be being mixed with 6 flavour among them more apparent (P ＜ 0.01); and sweet arenaceous team of 6 gentleman ball is compared, 6 flavour and in medicinal powder each dosage group is shrinking path of bowel of smallish rat complete stomach advances athletic time aspect to have significance difference (P ＜ 0.01) . And 6 flavour and in medicinal powder group and group of each lienal empty model are compared, have heighten GAS of lienal empty bandicoot, MTL and the effect that reduce VIP (P ＜ 0.01) , among them 6 flavour and in medicinal powder high dose group is more apparent (P ＜ 0.01) . 6 flavour mix conclusion in medicinal powder have promote motivation of bowel of small rat stomach, adjust the action with disorder hormone of bowel of stomach of lienal empty bandicoot.
Purpose observation is unripe, fry definitely the pharmacodynamics action of pine torch, compare both difference. The method uses 4 chloridize carbon to incur loss of liver of small rat acute, determine; of content of serum ALT, AST measures Yiwen to think of La Zaizheng to often be mixed the character of excrement and urine that small intestinal of costive small rat advances distance; to observe costive small rat, defecate is preclinical with defecate amount. The result is unripe, fry definitely pine torch all has the distinct effect that protect liver, can reduce level of serum ALT, AST, both quite, fry definitely pine torch defends liver action strong at be born definitely pine torch; is unripe, fry definitely pine torch all can increase to be mixed normally the small intestine of costive small rat presses action, improve the character of excrement and urine of costive small rat, shorten the defecate of costive small rat is preclinical, increase defecate number, both compare action to comparative. Conclusion is unripe, fry definitely pine torch all has protect liver and embellish bowel aperient action. Protecting liver to fall enzymatic respect, fry definitely pine torch is strong at be born definitely pine torch; is in embellish bowel is aperient respect, unripe definitely pine torch and fry definitely pine torch action comparatives.
Polysaccharide of purpose observation the root of remembranous milk vetch (total glucoside of Astragalus Polysaccharide) and root of herbaceous peony (the fat inside cell of bubble of sex of source of cell of huge to THP-1 bite affects Total Glucosides Of paeony) and its conpatibility of medicines character, from restrain the fat inside huge bite cell to collect angle to discuss Chinese traditional medicine character of active ingredient fight mechanism of sclerosis of arterial congee appearance. Methodological application microscope is photographed analyse software to measure a quantity to be fostered without ApoA-I surely partly like technology and graph base / ApoA-I is fostered base in the content with the qualitative fat inside cell of bubble of sex of source of cell of THP-1 huge bite. The result does not have ApoA-I the cholesterol inside cell of bubble of group of group of the root of remembranous milk vetch in education radical, conpatibility of medicines area ratio of fat qualitative coloring contrasts group of apparent increase (P ＜ 0.05);apoA-I is fostered base in cholesterol of group of group of root of herbaceous peony, conpatibility of medicines area ratio of fat qualitative coloring contrasts the group is apparent decrease (P ＜ 0.05) . Polysaccharide of conclusion the root of remembranous milk vetch can enhance lipoprotein of pair of oxidation low density gobble up ability, total glucoside of root of herbaceous peony can promote outflow of the cholesterol inside the cell. Conpatibility of medicines of total glucoside of polysaccharide of the root of remembranous milk vetch and root of herbaceous peony (12 ∶ 1) has fight action of sclerosis of arterial congee appearance potentially.
The purpose observes cassia twig naphtha (The Volatile Oil Of Ramulus Cinnamomi, of VORC) fight phlogistic action. The method uses side piece law, blood capillary connects law of the hyperfunction that appear a gender, sufficient strut law and bandicoot acute pneumonic model observes VORC fights phlogistic action. Result VORC connects fork of the hyperfunction that appear a gender, horn to the blood capillary of be caused by of the auricle strut of xylene be caused by, acetic acid sufficient strut of bandicoot of dish glue be caused by and coliform organisms endotoxin (the inhibition that pneumonic Dou Youming shows acute of bandicoot of LPS) be caused by. Conclusion VORC has fight phlogistic action significantly.
The purpose observes Xiexia bead medicine made of two or more ingredients is right the proliferous inhibition outside body of cell of HePG2 of person liver cancer discusses its action mechanism. The method is used 4 methylic occasionally law of azotic Zun blue colorimetric (MTT) and grow curvilinear mensuration, compound of Xie Xiazhu of more different pH indicator is opposite in different motive time the influence; of the HePG2 cell hyperplasia that fosters outside body applies art of cell drifting type, fluorescent microscope and electric lens to die to the wither of HePG2 cell at the same time rate and morphology have observation. As a result Xiexia bead compound has inhibition to HePG2 cell hyperplasia, inside certain limits, the chroma of Xiexia bead compound jumps over time of big, effect to grow more, stronger to the inhibition of HePG2, when chroma of Xiexia bead compound is 72 H of action of 500 μ G/mL, its are restrained rate amount to 93.58 ﹪ , IC50 is 240 μ G/mL. Compound of Xie Xiazhu of different pH indicator all has fixed rate the action that wither of revulsive HePG2 cell dies. Can certain outside body level restrains compound of conclusion Xie Xiazhu cell of cancer of the liver is proliferous, its mechanism may die with wither of cell of HePG2 of revulsive liver cancer about.
The influence of hyperplasia of adipose cell 3T3-L1 and become divided before the purpose observes shaddock skin glucoside is right, the possibility that discusses its to affect 3T3-L1 to split up action mechanism. The method fosters 3T3-L1 cell, use different pH indicator shaddock skin glucoside undertakes intervention, with 4 methylic occasionally azotic Zun salt (MTT) law detects the cell is proliferous, analyse the differentiation degree of adipose cell with coloring of oily red O and coloring colorimetric law. Changeover collection gets together more enzymatic couplet reacts (RT-PCR) detects gene crosses activation of content of oxide enzymatic hyperplasia to suffer body γ 2(PPAR related adipose cell become divided γ 2) , CCAAT/ increases child combinative albumen α (C/EBP α ) the expression of MRNA. Glucoside of result shaddock skin can restrain the hyperplasia of adipose cell before 3T3-L1 and become divided, can restrain PPAR γ 2, the expression of C/EBP α gene. Glucoside of conclusion shaddock skin can restrain the hyperplasia of adipose cell before 3T3-L1, can split up through reducing the expression of relevant gene restrains differentiation.