The purpose undertakes study to the chemical composition of chickweed. The method such as methodological coloring chart undertakes depart rarefied, change property and structure of spectral data appraisal according to manage. The result departs and appraisal 7 compound, it is Chinese rhubarb element respectively (Ⅰ ) , aether of armour of Chinese rhubarb element (Ⅱ ) , chinese rhubarb element - 8- armour aether (Ⅲ ) , 26 alkyl are mellow (Ⅳ ) , β - cereal steriod is mellow (Ⅴ ) , carrot glucoside (Ⅵ ) , hill Nai is phenolic - 3, 7-O- α - glucoside of L- buckthorn candy (Ⅶ ) . Ⅰ ～ Ⅳ and Ⅶ all are conclusion compound to share from inside this plant first, in belonging to a plant for chickweed, depart first get.
The purpose discusses element of Mongolian oak skin the effect of prevention and cure that chronic to animal experiment sex kidney declines. The method gives experimental bandicoot raise to caused chronic kidney in order to contain gland purine feed to decline in Feburary Ig of; interpose group gives Mongolian oak Pi Su (cure of 42d) of × of D-1 of · of 100mg · Kg-1. The essence when observing condition of health of each groups of animals and mortality; end says surviving bandicoot weight, kidney is weighed and law of drying of; of computational kidney index determines pathology of kidney of travel of groovy method of; of kidney organization water content checks; with Beckman full automatic biochemical analyzer determines serum kidney result is relevant biochemical index. Result and kidney decline contrast group quite, mortality of interpose group animal is reduced significantly () of ﹪ of 20 ﹪ Vs84, surviving rat weight increases significantly (＜ 0.001) of 14)g(P of ± of 240 ± 22)g vs(109, nephritic index and kidney organization water content all are reduced significantly [(﹪ of 1.25) of Vs(78.24 ± of ﹪ of 5.08 ± 0.89) and (﹪ of 0.90) of Vs(79.29 ± of ﹪ of 12.63 ± 2.94) ] (P ＜ 0.001, p ＜ 0.05) , change of nephritic pathology injury is apparent lighter, related serum kidney result biochemical index (BUN, CREA, URIC and GLU) change are reduced significantly [(20.9 ± 5.7) , (82.8 ± 51.5) , (155.9 ± 24.3) , (14.0) of ± of 11.79 ± 1.54)vs(78.7, (246.7 ± 55.9) , (108.7 ± 31.5) , (8.14 ± . Element of skin of conclusion Mongolian oak is chronic to bandicoot experiment sex kidney declines have effect of remarkable prevention and cure.
Composition of cholic acid of inject fluid Central Africa is opposite purpose research Qing Kailing cholic acid (CA) and different deoxygenate cholic acid (the influence that acting kinetic process reachs HDCA) medicine to distributing in main internal organs of the body, open the pharmacodynamics action of quick injection fluid in order to clarify clear. Methodological test animal divides 2 groups, qing Dynasty opens quick injection fluid (the CA/HDCA form that group of 10ml · Kg-1) and sheet use same dose, small rat is abstinence before giving drug can’t help water 12h, orbit minim collects blood 20 μ L, after oscillation of direct precipitation albumen centrifugal, take Qing Dynasty to detect into appearance. Metage of filter paper blot is used after taking heart, lung, kidney, head to clean, use 4 divide evenly of 1 ∶ oar hind is centrifugal take Qing Dynasty, the processing technique detects into appearance with hematic sample. Color atlas of use fluid photograph / usage of mass spectra couplet detects blood and the content that organize the CA in sample and HDCA. Result CA and HDCA are mixed in complete blood the limits of medicaments chroma linear in the organization is L-1 of · of μ G of 4 ～ 4 000, linear relationship is good (R ＞ 0.999) , lowest detects chroma is L-1 of · of 2 μ G, daytime and the RSD of the precision inside day all ＜ 5 ﹪ . Acting kinetic parameter mixes the drug that compares the CA when medicine made of two or more ingredients counteracts sheet to use and HDCA basically organizing (heart, lung, kidney, head) distributing mediumly, β of the AUC that Qing Dynasty opens the CA in quick injection fluid and HDCA, MRT, T1/2, V all when sheet of apparent prep above is used (P ＜ 0.05) , when CL is used under sheet apparently (of the CA in fluid of inject of Qing Kailing of P ＜ 0.05); and HDCA distributing apparent excel sheet uses CA/HDCA, CA and HDCA are in the distributinging characteristic in the organization: CHead of C of ＞ of heart of C of ＞ of kidney of lobar ＞ C. Conclusion this methodological sensitivity is tall, particularity is good, composition of cholic acid of Central Africa of fluid of inject of Qing Kailing of; of research of the process inside the body that applies to cholic acid apperception to close matter can lengthen cholic acid composition the distributinging;CA in the organization and HDCA are in the detention time inside body and composition of stimulative cholic acid low specification of the chroma in the head its pass through hematic head not easily.
The purpose is built Chinese the hemolysis of fluid of inject of element of armour of the root of fangji and agglomeration examination law. Law of the tube outside methodological body is used ” Chinese pharmacopoeia ” hemolysis of ” of rudder of application of law of injection security examination and agglomeration check 2005 edition Chinese traditional medicine law ” , consult bureau of supervisory processing of national provision medicines and chemical reagents is made ” excitant, irritability and haemolysis sex study chemical medicaments instructor principle ” relevant content. The result is 5.0mg · Ml-1 when chroma, when bulk is 0.3ml, inside 37 ℃ 3h, chinese incorrect hare red blood cell produces juice of inject of element of armour of the root of fangji haemolysis and agglomeration action. Conclusion can choose chroma to be 5.0mg · Ml-1, bulk is 0.3ml Chinese solution of sodium chloride of physiology of fluid of inject of element of armour of the root of fangji, the method that presses a regulation undertakes haemolysis and agglomeration are checked.
Water of root of beans of purpose research north carries content to be opposite velar fluidity and blood affect small rat red blood cell groovily. The method gives chroma to be respectively to 3 groups of small rat 1, 0.5, the water of boreal beans root of 0.25g · Ml-1 promotes thing, give medicine 3 weeks continuously, set group of solution of physiology sodium chloride to regard as contrast. Use hematic cell analyzer to undertake haemal composition determines, use law of DPH fluorescent bougie to determine fluidity of film of red blood cell. Result blood is groovy water of root of beans of the north in detecting promotes thing 3 dosage group, compare leucocyte amount with group of solution of physiology sodium chloride (WBC) has downtrend, chroma group has volume of cell of average blood of significance difference; in (content of MCV) , haemoglobin (HGB) reduces; significantly low, medium chroma group red blood cell (RBC) has ascendant tendency, low density group has plaque of statistical difference; (PLT) lifts significantly (P ＜ 0.01) . Boreal beans root is low, medium chroma team can add liquidity of film of red blood cell significantly, reduce small viscosity, and the effect of high concentration group is not apparent. Conclusion is certain water of root of beans of pH indicator north carries content to be able to add liquidity of red thin film, reduce haemal viscosity thereby, improve hematic current to change index.
The purpose builds law of color atlas of concentrated juice photograph to determine the method of content of acerbity ester of ammonia of pleasant of element of mildew of sulphone of hydrochloric acid armour. The method is efficient chromatographic column of; of law of fluid photograph color atlas: XDB-C18 column(250mm × 4.6mm, photograph of flow of 5 μ M); : 0.2 ﹪ phosphoric acid aqueous solution of 2 hydrogen ammonium - second nitrile (85 ∶ 15); detects wavelengh: 224nm. Result linear limits: 0Ml-1 of · of 1.004 6mg of.000 980 ～ , r=0.999 9; reclaims on average rate for 100.4 ﹪ , RSD is conclusion of 0.27 ﹪ ; this method result is accurate, the operation is handy, the content that applies to this medicaments measures calm analysis; to use HPLC method the influencing factor of stability of aqueous solution of acerbity to ammonia of pleasant of element of mildew of sulphone of hydrochloric acid armour ester undertakes study, the PH of solution is worth ammonia of pair of pleasant of element of mildew of sulphone of hydrochloric acid armour impact of stability of acerbity ester aqueous solution is the biggest, the stablest PH range is PH1 ～ 3.
The much delay of horse of hydrochloric acid music that purpose preparation day takes 2 times Shi Wei bolus. The method chooses Eudragit NE 30D to serve as Bao Yihuan commentate material, use preparation of law of the drug on solution of the gush end Glatt fluid-bed to carry medical tiny ball, hand-in-hand travel delay commentate Bao Yi. Shi Wei bolus is in the delay that makes as a result 1, 4, of 8 h release lead the 33 ﹪ of 30 ﹪ ～ that are indicative quantity respectively, 83 ﹪ of 66 ﹪ of 60 ﹪ ～ and 80 ﹪ ～ , the in releasing behavior to accord with traditional Chinese medicine of medicaments is in charge of 2005 edition are right this delay commentate preparation releases spent relevant provision. Conclusion studies craft is handy originally, reappearance is good, hopeful has industrialized production.
Beautiful element of small cup of purpose observation lamp injures bandicoot study to remember function and cerebral oxygen to traumatic sex head the influence of freedom radical. The method injures a law to build head of bandicoot scar sex to injure a model through hydraulic pressure, labyrinthian experiment mixes water avoid dark experiment determines bandicoot study remembers a function, always exceed oxide branch to change at the head is being taken to determine after the test enzymatic (T-SOD) , third 2 aldehyde (MDA) content. The result is in Morris water is labyrinthian in the experiment, beautiful element of lamp small cup can shorten apparently cerebral scar bandicoot escapes preclinical; avoiding in dark experiment, beautiful element of lamp small cup can lengthen cerebral scar significantly bandicoot study memory is preclinical, reduce wrong number. Beautiful element of lamp small cup can reduce cerebral scar significantly content of MDA of bandicoot brain tissue and add T-SOD content significantly. Hua Suke of conclusion lamp small cup improves cerebral scar bandicoot study remembers a function, its action and restrain oxygen freedom radical reaction is concerned.
Phenol of third of purpose observation different gives medicine to be short of blood to bandicoot full head beforehand again area of CA1 of the sea horse after perfusion is revulsive model one oxidation nitrogen closes enzymatic (the influence that INOS) conveys, different third phenol is discussed to be opposite on molecular level the late cranial nerve that send a gender yuan mechanism of scathing protection action. The method uses Pulsinelli-Brierley4 hemal block to break legal system to have full head is short of hematic model. Method of chemistry of applied immunity organization and former cross technology detects the expression of INOS of area of sea horse CA1. The result is short of blood together again perfusion contrasts group of photograph comparing, expression of INOS of area of CA1 of sea horse of pretreatment group bandicoot reduces different third phenol apparently, surviving nerve yuan number increases apparently, difference has statistical sense (P ＜ 0.05 or P ＜ 0.01) . Phenol of conclusion different third may be conveyed through restraining INOS and be short of blood to the head again perfusion sends sexual nerve behindhand yuan injury has certain protective effect.
Purpose experiment observes grain of kidney of Du Can beneficial fights tired out pharmacodynamics action. The method swims again in small rat ligature model, often press next small rat model of oxidation of bandicoot of Wistar of anoxic model, the aged and the small rat kidney that cause by hydroxyl Niao on model of deficiency of yang, observed grain of kidney of Du Can beneficial fights tired out pharmacodynamics action respectively. Result test result makes clear, grain of kidney of Du Can beneficial can lengthen small rat ligature weigh natant time significantly, large dose group (the average ligature of 10g · Kg-1) weighs natant time is NS contrasts the 2 times; of the group is in often approach sodium lime bottle inside, can raise small rat anoxic endurance, large dose can extend small rat surviving time apparently (P ＜ 0.01) , lengthen surviving rate to be able to add the content of the SOD inside red blood cell of bandicoot of the aged Wistar apparently for 82.5 ﹪ ; , SOD is added rate for 75.6 ﹪ , showed stronger fight oxidation (P ＜ 0.01); is in experiment of deficiency of yang of small rat kidney, the effect of deficiency of yang of small rat kidney that can effective antagonism causes by hydroxyl Niao, the weight of spermary and deputy sexual organs increases apparently, raise the content of small rat of kidney deficiency of yang and ketone of spermary of normal bandicoot serum significantly. Grain of kidney of Du Can beneficial has verdict fight exhaustion, be able to bear or endure anoxic, fight action of the oxidation, deficiency of yang that fight kidney, be a kind fight preparation of compound of new drug of tired out Chinese traditional medicine effectively.