The biology that the purpose researchs apperception of toad steriod Xi to close matter changes child Chan poison it is clever (Bufotalin) dies to the hurried wither of BGC-823 cell action, preliminary discuss its action mechanism. Methodological MTT law detects law of coloring of cellular poisonous action, Hoechst and Annexin V coloring law detect cellular wither dies, law of immune print cross detects the Caspase-3 of splitting decomposition form. Its spirit is in result Chan poison the to BGC-823 cell noxiousness action when 1 μ Mol/L achieves 50 ﹪ above, morphology observation and appearance of cell drifting type detect its Ling Keming shows poison of result indication Chan the wither of stimulative BGC-823 cell dies, the expression of Caspase-3 is moved on. Conclusion Chan poison its spirit has apparent hurried wither to die to BGC-823 cell action, its action mechanism may die through Caspase-3 wither the way comes true.
May 31, 2008
November 29, 2007
November 20, 2007
The vein below bandicoot tongue gives poison of medical China Chan to match base medicine is moved learn to study Pharmacokinetic Studies Of Cinobufagin After Sublingual Iv Administration In Rats
The purpose builds China Chan poison to match base the HPLC of hematic drug pH indicator inside bandicoot body determines method, and according to hematic drug chroma - time data is calculated the medicine inside its body is moved learn parameter. The vein below methodological classics tongue injects respectively 0.251, 0.503, poison of 1.006 mg/kg Hua Chan matchs base, use RP-HPLC law to determine chroma of content of serum Chinese traditional medicine, move with 3P87 medicine learn software computation medicine to move learn parameter. Poison of result China Chan matchs base the kinetic process inside bandicoot body can use one level the 2 rooms of kinetic process are open model the model will describe. Tall, medium, low the main medicine of group of 3 different dosage is moved learn parameter to be respectively: T1/2 α : 0β of.483 0, 0.377 7, 0.272 3 H;t1/2: 4.418 9, 5.897 2, 2.468 2 H;V(c):2.512 0, 8.660 6, 27.937 8 L/kg;AUC:1· of G/(h of 2.197 0, 8.412 3, 2.9056 μ ML);CL:2H) of · of.049 7, 5.943 7, 34.416 6 L/(kg. The RP-HPLC that conclusion studies to build originally determines bandicoot vein inject is different poison of dosage China Chan matchs base hind method of experiment of hematic drug pH indicator is handy, fast, acute, in serum inside noninterference of source sex material determines, poison of Ke Weihua Chan matchs base clinical safety is reasonable provide a basis with medicine and preparation quality control.