Herbal Medicine

Socialistic essence compares abstraction by itself, add reality and academic existence to wear bigger difference, question of student thought understanding is more, make socialism substaintial talked education must obtain actual effect hard. Be aimed at this one actual, raised the study interest that from the history and reality 2 respects arouse a student, with ” definition standard ” and ” compare law ” to tell clear ” what is socialistic ” , from theory and practice two levels solve student thought to understand the education strategy of the problem, explored an efficient way to heighten education actual effect.

Medicine of doctor of traditional Chinese medicine is having broad market in the country, to get used to requirement of work of sanitation of rural medical treatment, the country will increase level of technology of medical treatment of worker of rural medical treatment to include a plan, by the government contributive undertake grooming [1] . The medicine in carrying out a country to fulfil management board of medicine of national traditional Chinese medical science further teachs a project, the medicine in be being developed adequately serves medium function in rural Medical Protection, combine me to save current situation of job of medicine of rural traditional Chinese medical science, at the beginning of 2005, the education of doctor of traditional Chinese medicine that my courtyard regards Jiangxi as to save wholesome hall to decide grooms base, developed traditional Chinese medical science of hospital of complete province villages and towns clinical technology backbone grooms the job.

The purpose discusses a kind new ginseng is rare black glucoside 20-O-(β - dextrose of D- Bi Nan) - 20(S)- protoplast joins glucoside of 2 mellow black (IH-901) is right the hyperplasia of cell of BEL-7402 of person liver cancer restrains the effect and revulsive wither to die action. Method with 0, 6.25, 12.5, 25, 50, 75, the IH-901 action of 100 μ Mol/L the BEL-7402 cell at the education outside body, detect through MTT law the inhibition that IH-901 grows to the cell, differ configuration of microscope observation cell changes, the fluorescent nucleus coloring of AO/EB observes wither dies cellular configuration, art of cell drifting type detects cellular cycle and cellular wither die. Result MTT experiment shows IH-901 restrains the growth of BEL-7402 cell, observe typical wither dies below the means; microscope that shows time and chroma to depend on configuration change and biochemical feature: Go to  to wade agglomeration of? nucleus chromatin, occurrence wither dies after the fluorescent nucleus coloring of small system;AO/EB, observe typical wither dies cellular; sheds type cell appearance to be able to detect to wither die peak, and the cell is in by block sluggish G0/G1 period. The means that conclusion IH-901 can die through revulsive cell wither restrains the growth of cell of BEL-7402 of person liver cancer effectively, IH-901 of glucoside of rare ginseng black may become a kind to fight medicaments of cancer of the liver potentially.

The purpose discusses craft of microtherm n/med the process of preparing Chinese medicine protein to earthworm water-solubility composition and dissolve bolt and the influence that resist tumor active. The n/med the process of preparing Chinese medicine of methodological earthworm uses tradition and low temperature two kinds of craft, microtherm n/med the process of preparing Chinese medicine uses liquid nitrogen fast and refrigerant use SDS-PAGE and ImageTool software with freeze-dry technology; , the difference of protein to the earthworm of craft of two kinds of n/med the process of preparing Chinese medicine composition undertakes qualitative bolt of the dissolve outside analysing law of flat of; application fibrin and system with ration experiments, qualitative the difference; application of the fine dissolve active that analyses craft of two kinds of n/med the process of preparing Chinese medicine with ration shows small observation and MTT way, proliferous to cell of person liver cancer is being restrained outside body of craft of two kinds of n/med the process of preparing Chinese medicine active undertakes qualitative with quantitative trade off study. The water-solubility albumen composition of earthworm of craft of n/med the process of preparing Chinese medicine of result low temperature is richer than craft of traditional n/med the process of preparing Chinese medicine, the albumen of SDS-PAGE take; of craft of tradition of several as apparent as chroma prep above the earthworm albumen fluid of craft of two kinds of n/med the process of preparing Chinese medicine all has cell of remarkable the liver cancer that fight a person and fine dissolve active, but the craft of tradition of two kinds of active and remarkable prep above of craft of microtherm n/med the process of preparing Chinese medicine. Conclusion is different craft of n/med the process of preparing Chinese medicine reachs his to fight tumor and dissolve bolt active to distinct effect; is in to composition of earthworm water-solubility albumen conserve the protein of earthworm kind the biology active respect of medical effect composition,

The purpose discusses total glucoside of the root of herbaceous peony to grow to tumour of small rat of HepA liver cancer the influence that reachs tumor cell wither to die and its mechanism. Method small rat of 60 healthy Kunming, divide 5 groups randomly, it is model group respectively, amine of annulus phosphor acyl (100 Mg/kg) group, total glucoside of the root of herbaceous peony (240, 120 mg/kg) group, amine of annulus phosphor acyl (total glucoside of 100 Mg/kg) the root of herbaceous peony (240 mg/kg) group. Duplicate liver cancer of small rat HepA transplants hypodermically after the model, the 2nd day of Ig gives drug, give medicine 7 d continuously, the 8th day puts to death, take tumor organization, computational curb tumour is led, appearance of cell drifting type detects tumor cell wither dies to lead and analyse cellular cycle, and immune group changes a law to detect wither dies the expression of relevant albumen Bcl-2 and Pax. Tall, small dose restrains total glucoside of result the root of herbaceous peony apparently the growth of tumour of small rat of HepA liver cancer wither of; tumour cell dies the index adds; to restrain tumor cell Go/G1 period scale, to S period the cell is changed, wither dies the expression of albumen of the Bcl-2 in leading heighten; to reduce tumor cell, raise the expression of albumen of the Bax in tumor cell apparently at the same time. Total glucoside of conclusion the root of herbaceous peony grows to tumour of small rat of HepA liver cancer have apparent inhibition, wither of cell of revulsive tumour dies, its are main mechanism and adjust Bcl-2 and Bax albumen expression are concerned.

The purpose discusses 1- hydroxyl - 2, 3, 3 armour of 5- oxygen radical (buccal hill) ketone (QGS) is right fat polysaccharide (the protective effect that LPS) causes lung of small rat acute to injure and its mechanism. The method uses the method of Ip LPS to build lung of small rat acute to injure a model. Detect lung index, enzymatic law detects bronchus and alveolar fill lotion (level of the NO in BALF) , western blotting law detects B of κ of nuclear transcribe factor restrains α of B- of albumen I κ , revulsive model synthesis of one oxidation nitrogen is enzymatic (INOS) and annulus oxygen add up to enzymatic Ⅱ (the expression of the albumen such as COX-2) , HE coloring observes lung organizes pathology change. The lung index of the small rat that group of result QGS 500 Mg/kg can reduce LPS to cause significantly (group of P ＜ 0.05).QGS 250, 500 mg/kg all can reduce LPS to deliver level of the NO in small rat BALF significantly, restrain rate achieved respectively 37 ﹪ and 48.1 ﹪ . At the same time QGS 500mg/kg team still can raise lung to albumen of α of B- of κ of the I in the organization conveys an amount and reduce INOS and COX-2 albumen to convey an amount apparently. The loss of lung of small rat acute that conclusion QGS causes to LPS has protective effect, this action and the expression that its increase to albumen of α of I κ B- is conveyed and restrain INOS and COX-2 albumen are concerned.

The purpose discusses annulus dextrin bag to join the interaction rule between the deliquescent action with mellow to yew content and annulus dextrin and medicaments. The method inspects β - annulus dextrin and hydroxide propyl - β - annulus dextrin combines material for the bag solubilizing to yew alcohol when the influence;1H-NMR of action discusses a bag to add up to content to form advocate the interaction that degree changes to move between guest member. As a result the bag adds up to content material to add up to content to form a dominant action to the bag, hydroxide propyl - β - annulus dextrin is solubilizing to yew alcohol effect is apparent excel β - annulus dextrin, and solubilizing action therewith is proportional increase and increase;1H-NMR to analyse indication hydroxide propyl - β - the effect of balmy annulus in annulus dextrin and yew mellow element is the most distinct. Conclusion hydroxide propyl - β - the solubility that annulus dextrin can raise alcohol of yew of medicaments of difficult dissolve sex effectively.

The purpose discusses technique of small engraft of needle of fingered citron bine, breed for what fingered citron does not have ill nursery stock lay a foundation. The method fosters citric seedling to use as below dark condition stock, it is scion with needle of fingered citron bine, use “T” glyph law to undertake cauline needle small engraft experiments, undertake study to affecting the factor of grafting survival rate. The outcome acquired young plant of small engraft of needle of fingered citron bine successfully, optimized the condition of small engraft: The citric seedling of?4 d of ∪ ∶ Si falling into oblivion is stock, grafting survival rate is higher; it is scion in order to take the tip of fingered citron bine of 4 Xie Yuan radical, the effect is better, after grafting survival rate can amount to small engraft of needle of bine of 65.2 ﹪ ; , parafilm film tangles outside cut, can raise survival rate apparently. Conclusion established the method of small engraft of needle of fingered citron bine, obtained grafting to survive seedling.

The purpose discusses nitrogen (N) , phosphor (P) , Potassium (K) is different deserve to handle live thing of pair of violet awl chrysanthemum than fertilization the effect that He Juju acid measures output. The method is different to N, P, K deserve to help advance somebody’s career than fertilization the crop of biology of violet awl chrysanthemum of processing and the quantity with acerbity chicory undertake determining mixing statistic. Results of result first time handles entire individual plant to fight quality each by arrive high low ordinal for N K, CK, P, N P, N, N P K, P K, K; variance analysis result makes clear, n K, CK, P, N P, N 5 the nonsignificant difference between processing, but 5 processing all handle difference twice with P K, K remarkable. The quantity with each acerbity to chicory processing affects not remarkable; the 2nd times sampling N, N K, P, N P manages to compare CK group respectively everywhere tall 47.7 ﹪ , 35.4 ﹪ , 33.8 ﹪ , 12.3 ﹪ , n P K, P K, K manages to compare CK respectively everywhere low result of variance analysis of; of 7.7 ﹪ , 10.8 ﹪ , 28.5 ﹪ shows N processing and CK difference are notable, k processing is remarkable under CK. Conclusion considers to make clear different match fertilization the volume impact of acid of chicory of main to violet awl chrysanthemum active ingredient is not big; is right the influence of crop, n has dominant effect, p effect is not apparent, and potassium chloride causes reduction in production.

The purpose discusses β - the influence that function of film of phosphatide of cell of BIU-87 of cystic to the person cancer and Bcl-2 convey Lan sweet Xi. Method [32P]Pi isotope is joined, extraction cell phosphatide undertakes HPTLC analyses cellular phosphatide (PC and PE) metabolization, blue magic art of the Si Liang that take an examination of a horse determines level of cellular film albumen, fluidity of film of analytic cell fat and appearance of cell drifting type determine the change that Bcl-2 conveys. Result as β - pH indicator of Lan sweet Xi increases, [32P]Pi joins quantity of cellular PC, PE to be reduced apparently, velar albumen level and Bcl-2 expression are reduced apparently, its are the biggest restrain rate it is respectively: 41.1 ﹪ , 43.3 ﹪ , 43 ﹪ , 50 ﹪ , show the obvious concern that measure effect (P ＜ 0.05); β - Lan sweet Xi reduces liquidity of fat of BIU-87 cell film apparently. Conclusion β - Lan sweet Xi can control expression of metabolization of BIU-87 cell phosphatide, Bcl-2 and fat film liquidity apparently, clew phosphatide (PC and PE) and Bcl-2 and wither of cell of cystic cancer BIU-87 die have affinity.