Credit of Chinese pharmacy company evaluates FA-BP model and applied Development Of A Credit Scoring FA-BP Model In Chinese Pharmaceutical Market
Purpose: Core gnaws 糑 of small cup of ∮ of experienced ニ 562 cells wither dies the Caspase-3 in the process, 8, the trends of 9 active and MRNA changes, discuss its wither to die access. Method: 2Xin Fa of L-1 of · of 0 μ Mol cell of K562 of his lowing processing, 48 H observes cellular morphology;24, 48 with 72 h technology of cell drifting type detects cellular wither dies law of rate; colorimetric determines Caspase-3, 8 with 9 active; half mensurable RT-PCR detects Caspase-3, 8, the MRNA of 9 changes. Result: 2Xin Fa of L-1 of · of 0 μ Mol solid of occurrence nucleus of the cell after 48 H of cell of K562 of his lowing action shrinks, nuclear disintegrate and wither die the morphology such as small system changes;24, 48 with 72 h Xin Fa wither of cell of group of his lowing processing dies rate relatively contrast of the group increase a value to be respectively (﹪ of 6.1 ± 0.35) , (﹪ of 14.15 ± 0.42) and (﹪ of 30.7 ± 0.65) , difference all has significance (cut down of laborious of different time of P ＜ 0.01); the Caspase-3 of group of his lowing processing, 8, 9 active and MRNA expression quantity all are compared contrast the group is apparently elevatory (P ＜ 0.01) . Conclusion: CAspase-3, 8, the attune on 9 albumen active and MRNA is Xin Fa he lows the main reason that wither of revulsive K562 cell dies and mechanism.