Xiexia bead compound is restrained to the hyperplasia outside body of cell of HePG2 of person liver cancer reach revulsive wither to die action In -vitro Inhibitory Effect Of Phyllanthus Urinaria L Compound On Proliferation Of Human Liver Cancer Cell HePG2 And Its Apoptosis Induction
The purpose observes Xiexia bead medicine made of two or more ingredients is right the proliferous inhibition outside body of cell of HePG2 of person liver cancer discusses its action mechanism. The method is used 4 methylic occasionally law of azotic Zun blue colorimetric (MTT) and grow curvilinear mensuration, compound of Xie Xiazhu of more different pH indicator is opposite in different motive time the influence; of the HePG2 cell hyperplasia that fosters outside body applies art of cell drifting type, fluorescent microscope and electric lens to die to the wither of HePG2 cell at the same time rate and morphology have observation. As a result Xiexia bead compound has inhibition to HePG2 cell hyperplasia, inside certain limits, the chroma of Xiexia bead compound jumps over time of big, effect to grow more, stronger to the inhibition of HePG2, when chroma of Xiexia bead compound is 72 H of action of 500 μ G/mL, its are restrained rate amount to 93.58 ﹪ , IC50 is 240 μ G/mL. Compound of Xie Xiazhu of different pH indicator all has fixed rate the action that wither of revulsive HePG2 cell dies. Can certain outside body level restrains compound of conclusion Xie Xiazhu cell of cancer of the liver is proliferous, its mechanism may die with wither of cell of HePG2 of revulsive liver cancer about.